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411451

Sigma-Aldrich

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069)

liquid, clone HP6069, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.74

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

secondary antibodies

clone

HP6069, monoclonal

form

liquid

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

General description

Purified mouse monoclonal antibody. Recognizes human IgG1 Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) is validated for use in ELISA for the detection of Human IgG₁, Fc Fragment Specific.

Application

ELISA (see comments and application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial use aliquot and freeze (-20°C).

Other Notes

Can be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: 100%

Human IgG2: 0.1%

Human IgG3: 0.1%

Human IgG4: <0.02%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.01%

Human IgG2: <0.01%

Human IgG3: <0.01%

Human IgG4: <0.01%

Human IgA: <0.01%

Human IgE: <0.001%

Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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R G Keller et al.
Infection and immunity, 62(1), 215-220 (1994-01-01)
Previous studies found that normal human serum (NHS) contains an immunoglobulin G (IgG) antibody that mediates initiation of the classical complement pathway by nonencapsulated Cryptococcus neoformans. The present study used an enzyme-linked immunosorbent assay with whole nonencapsulated yeast cells as
Elizabeth H Aitken et al.
eLife, 10 (2021-06-29)
Plasmodium falciparum causes placental malaria, which results in adverse outcomes for mother and child. P. falciparum-infected erythrocytes that express the parasite protein VAR2CSA on their surface can bind to placental chondroitin sulfate A. It has been hypothesized that naturally acquired
D C Houpt et al.
Infection and immunity, 62(7), 2857-2864 (1994-07-01)
Serum obtained from normal human subjects contains antibodies reactive in an enzyme-linked immunosorbent assay with the glucuronoxylomannan (GXM) of Cryptococcus neoformans. The frequency of occurrence of class-specific antibodies among normal subjects was 28% for immunoglobulin G (IgG), 98% for IgM
H Lu et al.
Infection and immunity, 61(6), 2400-2407 (1993-06-01)
The aims of this study were to determine the immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b (Aab) for the different immunoglobulin (Ig) classes and subclasses and to determine the relative levels of these different Igs in serum. Seropositive early-onset periodontitis

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