345852
Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli
Geranylgeranyltransferase I, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.
Sinónimos:
GGTase I
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.54
Productos recomendados
recombinant
expressed in E. coli
Quality Level
assay
≥90% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn2+ for optimal activity. The enzyme is purified with Zn2+ tightly bound, so addition of Zn2+ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.
Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ for optimal activity.
Biochem/physiol Actions
1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
Warning
Toxicity: Standard Handling (A)
Physical form
In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Kalinin, A., et al. 2001. Protein Expr. Purif.22, 84.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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Shahar Molshanski-Mor et al.
Methods in molecular biology (Clifton, N.J.), 412, 385-428 (2008-05-06)
The superoxide (O2-)-generating enzyme complex of phagocytes, known as the NADPH oxidase, can be assayed in a number of in vitro cell-free (or broken cell) systems. These consist of a mixture of the individual components of the NADPH oxidase, derived
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