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217721

Sigma-Aldrich

RO-3306

≥95% (HPLC), liquid, Cdk1 inhibitor, Calbiochem®

Sinónimos:

InSolution Cdk1 Inhibitor IV, RO-3306, 5-(6-Quinolinylmethylene)-2-((2-thienylmethyl)amino)-4(5H)-thiazolone, 5-(Quinolin-6-ylmethylene)-2-(thiophen-2-ylmethylamino)thiazol-4(5H)-one, 5-(Quinolin-6-ylmethylene)-2-(thiophen-2-ylmethylamino)thiazol-4(5H)-one, 5-(6-Quinolinylmethylene)-2-((2-thienylmethyl)amino)-4(5H)-thiazolone

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About This Item

Fórmula empírica (notación de Hill):
C18H13N3OS2
Peso molecular:
351.45
MDL number:
MFCD17392573
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Cdk1 Inhibitor IV, RO-3306, InSolution, ≥95%

Quality Level

100

assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
desiccated (hygroscopic)
protect from light

shipped in

dry ice

storage temp.

−70°C

General description

A cell-permeable quinolinyl thiazolinone compound that acts as a potent and ATP-competitive inhibitor of Cdk1 (Ki = 35 nM and 110 nM for Cdk1/B1 and Cdk1/A, respectively). It affects Cdk2/E, PKCδ, and SGK only at much higher concentrations (Ki = 340, 318, and 497 nM, respectively), and shows little effect against Cdk4/D and six other commonly studied kinases (Ki =2 M). Short-term treatment for up to 20 hrs results in fully reversible G2/M cell cycle arrest, while prolonged treament (>48 hrs) results in apoptotic cell death in proliferating cancer cells, but not in nontumorigenic epithelial cell lines. The solid form of this compound (Cat. No. 217699) is also available.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Cdk1/B1 and Cdk1/A
Reversible: yes
Secondary Target
Cdk2/E, PKCδ, and SGK
Target Ki: 35 nM and 110 nM for Cdk1/B1 and Cdk1/A,

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 10 mM (2 mg/569 L) solution of Cdk1 Inhibitor IV, RO-3306 (Cat. No. 217699) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Other Notes

Vassilev, L.T., et al. 2006. Proc. Natl. Acad. Sci. USA103, 10660.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

188.6 °F - closed cup - (refers to pure substance)

flash_point_c

87 °C - closed cup - (refers to pure substance)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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