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208713

Sigma-Aldrich

Calpain-1, Human Erythrocytes

Calpain-1, Human Erythrocytes, is a native calpain-1. A heterodimeric cysteine proteinase with low Ca2+ requirement (EC₅₀ = 2 µM).

Sinónimos:

μ-Calpain

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About This Item

Comisión internacional de enzimas:
Código UNSPSC:
12352202
NACRES:
NA.77

Nivel de calidad

Formulario

liquid

actividad específica

≥1000 units/mg protein

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

Condiciones de envío

wet ice

temp. de almacenamiento

−70°C

Descripción general

Native calpain-1 from human erythrocytes. Ca2+-dependent cysteine proteinase with low Ca2+ requirement (half-maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin.

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Harmful (C)

Definición de unidad

One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 1 fluorogenic unit.

Forma física

In 20 mM imidazole, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.

Nota de preparación

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Nota de análisis

Comparable to reference lot by SDS-PAGE

Otras notas

Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol.81, 323.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Croall, D.E., and McGrody, K.S. 1994. Biochemistry33, 13223.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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