17-10109
ChIPAb+ TCF-4 - ChIP Validated Antibody and Primer Set
from mouse
Sinónimos:
HMG box transcription factor 4, Transcription factor 7-like 2, T-Cell factor 4, T-cell-specific, HMG box, T-Cell-specific transcription factor 4, Transcription factor 7-like 2
About This Item
Productos recomendados
biological source
mouse
Quality Level
clone
monoclonal
species reactivity
human, mouse
manufacturer/tradename
ChIPAb+
Upstate®
technique(s)
ChIP: suitable
dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
GenBank accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... TCF7L2(6934)
Categorías relacionadas
General description
The ChIPAb+ TCF-4 set includes the TCF-4 antibody, a negative control normal mouse IgG, and qPCR primers which amplify a 198 bp region of human SP5 promoter. The TCF-4 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of TCF-4-associated chromatin.
Specificity
Immunogen
Application
Representative lot data.
Sonicated chromatin prepared from HT29 cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of Normal Mouse IgG or 2 µg Anti-TCF-4 and the Magna ChIP® G Kit (Cat. # 17-611).
Successful immunoprecipitation of TCF-4 associated DNA fragments was verified by qPCR using ChIP Primers, SP5 Promotor as a positive locus, and GAPDH promoter primers as a negative locus (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP G (Cat. # 17-409) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Cos-1 cells were transfected with TCF-4 cDNA in pUSE (lane 2) or pUSE (lane 1). RIPA lysates were prepared, resolved by electrophoresis, transferred to nitrocellulose and probed with anti-TCF-4 (1 mg/mL).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates TCF-4 (~66 kDa) (Please see figures).
Immunocytochemistry/Immunohistochemistry:
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).
Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with Beta-Catenin (Persad, S., 2001).
Supershift Assay (EMSA):
A previous lot of this antibody was reported to supershift TCF-4/DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).
Epigenetics & Nuclear Function
Transcription Factors
Packaging
Quality
Sonicated chromatin prepared from HT29 cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of Normal Mouse IgG or 2 µg of Anti-TCF-4 and the Magna ChIP® G Kit (Cat. # 17-611).
Successful immunoprecipitation of TCF-4 associated DNA fragments was verified by qPCR using ChIP Primers, SP5 Promotor (Please see figures).
Please refer to the EZ-Magna ChIP G (Cat. # 17-409) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Target description
Physical form
Normal Mouse IgG. Two vials containing 25 µg of purified IgG in 25 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, SP5 promote. One vial containing 75 μL of 5 μM of each primer specific for human SP5 promotor. Store at -20°C.
FOR: GGG TCT CCA GGC GGC AAG
REV: AGC GAA AGC AAA TCC TTT GAA TCC
Storage and Stability
Analysis Note
Includes negative control normal mouse IgG and primers specific for human SP5 promoter.
Legal Information
Disclaimer
Storage Class
10 - Combustible liquids
Certificados de análisis (COA)
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