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Merck
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Key Documents

05-185

Sigma-Aldrich

Anti-avian Src Antibody, clone EC10

clone EC10, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

EC10, monoclonal

species reactivity

avian

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_005417.3
NM_198291.1

UniProt accession no.

P12931

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SRC(6714)

Specificity

specific only for avian Src; this antibody is excellent for distinguishing pp60src in cells transfected with v-src or c-src expression vectors.

Immunogen

Recombinant chicken pp60src

Application

Anti-avian Src Antibody, clone EC10 detects level of avian Src & has been published & validated for use in IP, WB & IC.

Quality

routinely evaluated in (60kDa) on a RIPA lysate of chicken embryo fibroblasts (CEF)

Target description

60 kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, with 0.05% sodium azide
Format: Purified

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Normal cells control the growth of neighboring transformed cells independent of gap junctional communication and SRC activity.
David B Alexander, Hitoshi Ichikawa, John F Bechberger, Virginijus Valiunas, Misao Ohki et al.
Cancer Research null
Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene.
Reynolds, A B, et al.
Molecular and cellular biology, 9, 629-638 (1989)
Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
Toyli, M; Rosberg-Kulha, L; Capra, J; Vuoristo, J; Eskelinen, S
Laboratory Investigation; a Journal of Technical Methods and Pathology null
Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin
Parsons, S. J., et al
Journal of virology, 51, 272-282 (1984)
Wanping Xu et al.
Molecular cell, 47(3), 434-443 (2012-06-26)
Many critical protein kinases rely on the Hsp90 chaperone machinery for stability and function. After initially forming a ternary complex with kinase client and the cochaperone p50(Cdc37), Hsp90 proceeds through a cycle of conformational changes facilitated by ATP binding and
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