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Avanti

E. coli Extract Total

Avanti Research - A Croda Brand

Sinónimos:

E. coli Total Lipid Extract

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
12352211
NACRES:
NA.25

Formulario

powder

envase

pkg of 1 × 1 g (100500P-1g)
pkg of 1 × 100 mg (100500P-100mg)
pkg of 5 × 100 mg (100500P-500mg)

fabricante / nombre comercial

Avanti Research - A Croda Brand

tipo de lípido

lipid extracts

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Aplicación

E. coli Extract Total has been used in the preparation of unilamellar vesicles in lipid screening experiments of membrane protein green fluorescent protein (GFP) based fusion proteins and in the reconstitution of proteoliposomes.

Acciones bioquímicas o fisiológicas

The E. coli lipid extract is a combination of 16, 17 and 18 carbon containing phospholipids namely, phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. It is used for preparing supported lipid bilayers. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in identification and taxonomic grouping of any bacterial sample.

Envase

5 mL Clear Glass Sealed Ampule (100500P-100mg)
5 mL Clear Glass Sealed Ampule (100500P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100500P-1g)

Información legal

Avanti Research is a trademark of Avanti Polar Lipids, LLC

también adquirido normalmente con este producto

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins
Nji E, et al.
Nature Communications, 9(1), 4253-4253 (2018)
Membrane insertion of small proteins
Protein Secretion, 39-62 (2010)
The extracellular gate shapes the energy profile of an ABC exporter
Hutter CAJ, et al.
Nature Communications, 10(1), 2260-2260 (2019)
Denis G Knyazev et al.
Biomolecules, 10(1) (2020-01-18)
The bacterial channel SecYEG efficiently translocates both hydrophobic and hydrophilic proteins across the plasma membrane. Translocating polypeptide chains may dislodge the plug, a half helix that blocks the permeation of small molecules, from its position in the middle of the
Bettina Zens et al.
Methods (San Diego, Calif.), 75, 37-43 (2014-12-03)
Macroautophagy is a major bulk degradation pathway for cytoplasmic material in eukaryotic cells. During macroautophagy, double membrane-bound organelles called autophagosomes are formed in a de novo manner. In the course of their formation autophagosomes capture cytoplasmic material, which is subsequently

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