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Key Documents

TEM-AUFT313-05

Sigma-Aldrich

Au-Flat R 1.2/1.3

Gold Grids, size 300 mesh, pack of 5

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About This Item

UNSPSC Code:
41111761
NACRES:
MA.24

packaging

pack of 5

size

300 mesh

General description

Come with 1.2 μm/1.3 μm hole pattern on 300 mesh Gold grids. Au-Flat features a holey Au/Pd film on a gold mesh grid, it′s chemically inert and biologically compatible. Au-Flat is an ultrastable Cryo-EM sample support with a 45nm holey Gold alloy film on 3 mm gold mesh grids. Au-Flat is a derivative of the C-Flat product.Au-Flat improves image quality and resolution with the gold alloy film, which reduces particle movement during imaging and significantly reduces beam-induced motion during imaging compared to carbon films. Au-Flat film is significantly stronger than carbon films and is more capable of surviving the Cryo-EM workflow including tweezer handling, glow discharge, blotting, auto-grid loading and plunge freezing.Benefits over other Gold Foil TEM Grids:Fewer Mistakes The lighter color of the Au/Pd alloy film compared to the underlying gold mesh grid makes it easy to identify the "holey" side of the grid during sample prep. This ensures the sample is deposited on the correct side and that the grid is loaded and imaged in the correct orientation.Stronger Au-Flat is produced with gold mesh grids that are about 6 microns thicker than typical Cryo-EM grids. This makes the grids stiffer and less likely to bend or deform. The increased thickness is fully compatible with side-entry holders as well as auto-loaders.

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