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ZRB1603

Sigma-Aldrich

Anti-RUNX2 Antibody, clone 1D6 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):

Runt-related transcription factor 2;Acute myeloid leukemia 3 protein;Core-binding factor subunit alpha-1;CBF-alpha-1;Oncogene AML-3;Osteoblast-specific transcription factor 2;OSF-2;Polyomavirus enhancer-binding protein 2 alpha A subunit;PEA2-alpha A;PEBP2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1D6, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 56.65 kDa
observed mol wt ~58 kDa

purified by

using Protein A

species reactivity

human, mouse

species reactivity (predicted by homology)

monkey, feline, canine, bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

Internal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D6 is a Rabbit recombinant monoclonal antibody that specifically detects Runt-related transcription factor 2 (RUNX2). It targets an epitope within 23 amino acids from the internal region

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of human Runt-related transcription factor 2 (RUNX2).

Application

Quality Control Testing

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RUNX2 in C2C12 cell lysate.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RUNX2 in SAOS-2 cella.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RUNX2 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound RUNX2 with a KD of 1.7 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Runt-related transcription factor 2 (UniProt: Q13950; also known as Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, CBF-alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirus enhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, RUNX2) is encoded by the RUNX2 (also known as AML3, CBFA1, OSF2, PEBP2A) gene (Gene ID: 860) in human. RUNX2 is a master transcription factor that is specifically expressed in membranous and endochondral bones and is involved in osteoblastic differentiation and skeletal morphogenesis. Its expression in the bone and osteogenic front of a suture is crucial for cranial suture closure and membranous bone morphogenesis. It is a heterodimer of a and b subunits. The a subunit binds DNA as a monomer through its RUNT domain (aa 101-229). Its activity is shown to be precisely regulated by phosphorylation at multiple sites. Protein kinase C activated by FGF2 phosphorylates RUNX2 at serine 247 and ERK phosphorylates serine 301 in the PST domain that is reported to be critical for the enhancement of subsequent acetylation and the suppression of ubiquitination of the RUNX2 protein. Phosphorylation of serine 451 by CDK1 is reported to promotes endothelial cell proliferation. Mutations in RUNX2 gene have been linked to craniofacial malformations. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kim, WJ., et al. (2020). Exp. Mol. Med. 52(8); 1178-1184; Kawane, T., et al (2018). Sci. Rep. 8; Article 13551).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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