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Key Documents

SAB4504099

Sigma-Aldrich

Anti-phospho-PKC-pan (pThr497) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-PKCL, Anti-PRKCL, Anti-nPKC-eta

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 76 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:40000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P05129
P05771
P17252
P24723
Q02156
Q04759
Q05513
Q05655

UniProt accession no.

P24723

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr497)

Gene Information

human ... PRKCA(5578) , PRKCB(5579) , PRKCD(5580) , PRKCE(5581) , PRKCG(5582) , PRKCH(5583) , PRKCQ(5588) , PRKCZ(5590)

General description

Protein kinase C is a member of the cAMP-dependent, cGMP-dependent, and protein kinase C (AGC) superfamily of protein kinases. It has more than14 isoforms. A catalytic domain with an ATP binding site and a substrate-binding site is found in all PKC isoforms.

Immunogen

The antiserum was produced against synthesized peptide derived from human PKC-pan around the phosphorylation site of Thr497.

Immunogen Range: 623-672

Application

Anti-phospho-PKC-pan (pThr497) antibody produced in rabbit has been used in western blot analysis (1:500).

Biochem/physiol Actions

Protein kinase Cs (PKCs) are involved in the growth and function of the gastrointestinal tract and the immune response. PKCs regulates cyclins, cyclin-dependent kinases (cdks), cdk inhibitors, and cell division cycle 25 (cdc25) phosphatases.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adrian R Black et al.
Frontiers in immunology, 3, 423-423 (2013-01-22)
A link between T cell proliferation and the protein kinase C (PKC) family of serine/threonine kinases has been recognized for about 30 years. However, despite the wealth of information on PKC-mediated control of, T cell activation, understanding of the effects
Genevieve L Wojcik et al.
mBio, 11(1) (2020-02-06)
Diarrhea is a major cause of both morbidity and mortality worldwide, especially among young children. Cryptosporidiosis is a leading cause of diarrhea in children, particularly in South Asia and sub-Saharan Africa, where it is responsible for over 200,000 deaths per
Serine and threonine phosphorylation
Plattner F and Bibb JA
Basic Neurochemistry, 467-492 (2012)
Shujie Ning et al.
International journal of molecular sciences, 20(7) (2019-04-17)
Previous studies have demonstrated that monochromatic light affects plasma melatonin (MEL) levels, which in turn regulates hepatic insulin-like growth factor I (IGF-I) secretion via the Mel1c receptor. However, the intracellular signaling pathway initiated by Mel1c remains unclear. In this study

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