Skip to Content
Merck
All Photos(3)

Key Documents

SAB4200878

Sigma-Aldrich

Anti-Escherichia coli O111:B4 LPS, Mouse monoclonal

clone Ecco14, purified from hybridoma cell culture

Synonym(s):

E.coli bacteria

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

antibody form

purified from hybridoma cell culture

Quality Level

clone

Ecco14

form

liquid

species reactivity

E. coli

concentration

~1 mg/mL

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. A typical LPS molecule has lipid A (a glucosamine-based phospholipid), a relatively short core oligosaccharide and a distal polysaccharide (O-antigen).

Specificity

Monoclonal Anti-Escherichia coli O111:B4 LPS antibody specifically recognizes LPS of O111:B4 E. coli subtype and has no cross reactivity with Escherichia coli O26:B6 LPS, Escherichia coli eh100 LPS, P. gingivalis, P. mirabilis, S. typhimurium, S. enteritidis.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA.

Biochem/physiol Actions

LPSs contribute to the integrity of the outer membrane and protect the cell against the action of bile salts and lipophilic antibiotics. They stimulate host cells via TLR2 and TLR4 (toll like receptors). This stimulation results in the generation of various proinflammatory cytokines, such as TNFα (tumor necrosis factor), IL-1 (interleukin 1) and IL-6.1,2 Host cells stimulation later leads to hepatic secretion of acute phase proteins (APP) such as serum amyloid A (SAA) and haptoglobin (Hp).3,4 Acute phase proteins are known biomarkers for the diagnosis of several inflammatory conditions.5,6

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service