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P5975

Millipore

Amino-terminal Met-FLAG-BAP™ Fusion Protein

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About This Item

MDL number:
MFCD01632954
UNSPSC Code:
12352202
NACRES:
NA.32

mol wt

~49 kDa

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Related Categories

Application

Amino-terminal Met-FLAG-BAP™ Fusion Protein is useful for assurance of the functional integrity of ANTI-FLAGÒ M2 and M5 monoclonal antibodies in immunological procedures such as Western blotting, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy and FACS.
Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Amino-terminal Met-FLAG-BAP™ control protein is a 468 amino acid N-terminal Met-FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular weight of 49.4 kDa. The Amino-terminal Met-FLAG-BAP control protein migrates as a 45-55 kDa band by SDS-PAGE depending on the electrophoretic conditions.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK0200
121K9079
098H9047
062K9075
039H9077

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Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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