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P2459

Sigma-Aldrich

Phenyl-Sepharose 6 Fast Flow

high substitution, extent of labeling: ~40 μmol per mL (high substitution)

Synonym(s):

Phenyl-Agarose

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About This Item

MDL number:
UNSPSC Code:
41106500
NACRES:
NA.56

Quality Level

form

suspension

extent of labeling

~40 μmol per mL (high substitution)

matrix

agarose, 6% cross-linked

matrix activation

epichlorohydrin

matrix attachment

ether

matrix spacer

3 atoms

particle size

45-165 μm

storage temp.

2-8°C

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General description

P2459-200ML′s updated product is GE17-0973-05

Application

Phenyl Sepharose is used in protein chromatography, affinity chromatography, hydrophobic interaction media, resins and separation media. Phenyl Sepharose has been used in studies that contributed to improving industrial applications in additives in detergents and feed industries. Phenyl Sepharose has also been used to study microbial communities inhabiting hypersaline environments.

Physical form

Suspension in 20% ethanol
aqueous ethanol suspension

Legal Information

Sepharose is a trademark of Cytiva

related product

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

104.0 °F

Flash Point(C)

40 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K A Rye et al.
The Journal of biological chemistry, 271(8), 4243-4250 (1996-02-23)
The effect of sphingomyelin (SPM) on the structure and function of discoidal and spherical reconstituted high density lipoproteins (rHDL) has been studied. Three preparations of discoidal rHDL with 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC)/SPM/unesterified cholesterol (UC)/apolipoprotein (apo)A-I molar ratios of 99.6/0. 0/10.2/1.0, 86.0/13.6/10.8/1.0
K A Rye et al.
The Journal of biological chemistry, 270(1), 189-196 (1995-01-06)
The effect of cholesteryl ester transfer protein (CETP) on the size, composition, and structure of spherical, reconstituted HDL (rHDL) which contain apolipoprotein (apo) A-I as their sole apolipoprotein has been studied. Spherical rHDL were incubated with CETP and Intralipid for
Development of a downstream process for the isolation of <I>Staphylococcus aureus</I> arsenate reductase overproduced in <I>Escherichia coli</I>.
Messens, J., et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 737, 167-178 (2000)
Purification and partial characterization of <I>Rhizomucor miehei</I> lipase for ester synthesis.
Wu, X.Y., et al.
Applied Biochemistry and Biotechnology, 59, 145-158 (1996)
C Mawadza et al.
Journal of biotechnology, 83(3), 177-187 (2000-10-29)
Cellulases produced by two Bacillus strains, CH43 and HR68, isolated from hot springs in Zimbabwe, were purified to homogeneity from culture supernatants. Both enzymes had molecular mass of 40 kDa and isoelectric point of 5.4. The enzymes also resembled each

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