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Key Documents

H6383

Sigma-Aldrich

Hydroxyalkoxypropyl-Dextran

Type IX

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About This Item

MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

type

Type IX

Quality Level

form

beads

technique(s)

liquid chromatography (LC): suitable

storage temp.

2-8°C

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Application

Hydroxyalkoxypropyl-Dextran is used for liquid chromatography, protein chromatography, and gel filtration chromatography. Hydroxyalkoxypropyl-Dextran has been used to develop a nondestructive method for effective removal of lipids from small quantities of aqueous protein as well as to develop a highly specific method for the determination of cortisol in human serum and urine.
Lipophilic, hydrophobic gel for liquid chromatography.

Other Notes

Hydroxypropyl beaded dextran (Lipophilic Sephadex, LH-20-100), substituted with long chain alkyl ethers.
Substituted approx. 50% by weight with alkyl chains of length C15-C18

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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U Kragh-Hansen
Analytical biochemistry, 210(2), 318-327 (1993-05-01)
A simple and nondestructive method was developed for effective removal of lipids, such as long-chain fatty acids and steroids, from small quantities (2-10 mg) of aqueous protein. The procedure operates with a high recovery of protein (97%) and was elaborated
J B Lowe et al.
The Journal of biological chemistry, 262(12), 5931-5937 (1987-04-25)
Rat intestinal fatty acid-binding protein (I-FABP) is an abundant, 15,124-Da polypeptide found in the cytosol of small intestinal epithelial cells (enterocytes). It is homologous to rat liver fatty acid-binding protein (L-FABP), a 14,273-Da cytosolic protein which is found in enterocytes
D Apter et al.
Clinica chimica acta; international journal of clinical chemistry, 63(2), 139-148 (1975-09-01)
A highly specific method for the determination of cortisol in human serum and urine is described. The sample is first extracted with diethyl ether/ethyl acetate (1 : 1, by vol.), then chromatographed on a highly lipophilic derivative of Sephadex (hydroxyalkoxypropyl

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