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DCL6B100

Sigma-Aldrich

DEAE–Sepharose™

CL-6B

Synonym(s):

Diethylaminoethyl–Sepharose™

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About This Item

CAS Number:
57407-08-6
MDL number:
MFCD00146630
UNSPSC Code:
47101511
NACRES:
NA.56

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm

pore size

~4,000,000 Da exclusion limit

pH

3—12

capacity

130-170 μeq/mL binding capacity (gel volume)(gel volume)

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General description

DCL6B100-500ML′s updated product number is GE17-0710-01

Application

DEAE-Sepharose® is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Legal Information

DEAE-Sepharose is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M A Heine et al.
Molecular biology of the cell, 4(11), 1189-1204 (1993-11-01)
Epitope-tagged Xenopus nucleolin was expressed in Escherichia coli cells and in Xenopus oocytes either as a full-length wild-type protein or as a truncation that lacked the distinctive carboxy glycine/arginine-rich (GAR) domain. Both full-length and truncated versions of nucleolin were tagged
J J Wheeler et al.
Gene therapy, 6(2), 271-281 (1999-08-06)
A detergent dialysis procedure is described which allows encapsulation of plasmid DNA within a lipid envelope, where the resulting particle is stabilized in aqueous media by the presence of a poly(ethyleneglycol) (PEG) coating. These 'stabilized plasmid-lipid particles' (SPLP) exhibit an
Preparative affinity precipitation of L-lactate dehydrogenase.
Pearson, J.C., et al.
Journal of Biotechnology, 11(2-3), 267-274 (1989)
M L Zapp et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(17), 7734-7738 (1991-09-01)
The Rev protein of human immunodeficiency virus type 1 is a sequence-specific RNA binding protein that is essential for viral replication. Here we present evidence that Rev is a stable oligomer both in vitro and in vivo. Analysis of Rev
M Vasseur
Bioscience reports, 9(3), 341-346 (1989-06-01)
The rabbit intestinal sucrase-isomaltase complex has been purified to homogeneity after solubilization with Triton X 100 followed by chromatography on DEAE Sepharose CL 6B and a second solubilization with papain. After hydrophobic chromatography on Octyl Sepharose CL 6B, separation from
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