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C5024

Sigma-Aldrich

Carbonic Anhydrase from bovine erythrocytes

non-denaturing PAGE marker

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

Quality Level

form

lyophilized powder

mol wt

~29 kDa

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

storage temp.

−20°C

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Application

This product is used as a marker to determine the molecular mass of an unknown protein in non-denaturing electrophoresis systems. In such systems, proteins often retain their biological activity, thus allowing in-gel detection using specific substrate stains. This technique also facilitates the recovery of the active protein. The product yields 3 bands.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A O Kaya et al.
Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 17(4), 663-668 (2013-01-22)
Hypoxia is common in many solid tumors such as breast, head-neck, and soft tissue malignancies. Hypoxia causes overexpression of hypoxia inducible factor-1 alpha (HIF-1α) and carbonic anhydrase IX (CA IX) which are associated with unfavorable prognosis in breast cancer. In
Tristan J Horner et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(7), 2500-2505 (2013-01-31)
The global marine distributions of Cd and phosphate are closely correlated, which has led to Cd being considered as a marine micronutrient, despite its toxicity to life. The explanation for this nutrient-like behavior is unknown because there is only one
Frédéric D Birkhäuser et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 36(2), 102-111 (2013-02-05)
The dendritic cell vaccine DC-Ad-GM·CAIX is an active, specific immunotherapy with the potential of providing a safe and effective therapy against renal cell carcinoma (RCC). Using immunocompetent Balb/c mouse models we tested the efficacy and mechanism of the vaccine to
Wolfgang Maret
Advances in nutrition (Bethesda, Md.), 4(1), 82-91 (2013-01-16)
The nutritional essentiality of zinc for the growth of living organisms had been recognized long before zinc biochemistry began with the discovery of zinc in carbonic anhydrase in 1939. Painstaking analytical work then demonstrated the presence of zinc as a
Peter Burghout et al.
Journal of bacteriology, 195(7), 1573-1582 (2013-01-29)
Although carbon dioxide (CO2) is known to be essential for Streptococcus pneumoniae growth, it is poorly understood how this respiratory tract pathogen adapts to the large changes in environmental CO2 levels it encounters during transmission, host colonization, and disease. To

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