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A5757

Sigma-Aldrich

Antifoam B Emulsion

aqueous-silicone emulsion

Synonym(s):

Antifoaming agent B

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About This Item

UNSPSC Code:
12161900
NACRES:
NA.25

Quality Level

form

emulsion

contains

emulsifier (different from those present in Antifoam A emulsion)

technique(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

General description

Antifoam eliminates excessive foaming formed during impingement, due to proteins and/or carbohydrates in the collection medium. Antifoam B emulsion is a silicone based antifoam. Antifoam B emulsion does not affect the growth of bacteria. Antifoam B emulsion functions as an antifoaming agent in shake flask culture of bacteria.

Application

Antifoam B emulsion has been used:
  • to enhance collection efficiency in collection media
  • as a component in stress granule lysis buffer to reduce foaming, in host cell lysis buffer to reduce foaming caused by detergents
  • to improve solubility of antibiotics
  • as a foaming controls in bacterial growth media

Other Notes

A 10% aqueous emulsion of polydimethylsiloxane

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

>214.0 °F - closed cup

Flash Point(C)

> 101.1 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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