MABT530

Anti-N-cadherin Antibody, clone 6A9.2

clone 6A9.2, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6A9.2, monoclonal
• species reactivity: rat, human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001783
• UniProt accession no.: P19022
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CDH2(1000)

Description

General description

Cadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. N-Cadherin is a single-pass type I membrane protein that plays a role in calcium-dependent cell adhesion. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. Cadherins are synthesized as precursor proteins that are proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis.

Specificity

Clone 6A9.2 targets an epitope within N-cadherin extracellular cadherin 1 domain.

Immunogen

GST/His-tagged recombinant human N-cadherin extracellular cadherin 1 domain fragment.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected N-cadherin in human cervical cancer, adrenal gland, and liver tissue sections.

Research Category
Cell Structure

This mouse monoclonal Anti-N-cadherin antibody, clone 6A9.2, Cat. No. MABT530 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of N-cadherin.

Quality

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-cadherin in 10 µg of C6 rat glioma cell lysate.

Target description

~140 kDa observed. Target band size appears larger than the calculated molecular weights of 99.81/97.23/82.03 kDa (prepro-/pro-/mature human N-cadherin) and 99.69/97.24/81.91 kDa (prepro-/pro-/mature rat N-cadherin) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: 04-1126

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1