AB1847

Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop

Chemicon^®, from rabbit

• Synonym(s): CD184, CXCR4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_001008540.1; NM_003467.2
• UniProt accession no.: P61073
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CXCR4(7852)

Description

Specificity

Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (Nimitrov 1997). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principle coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (Feng et al. 1996; Berson et al. 1996). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (Doranz et al. 1996; Endres et al. 1996). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (Bleul et al. 1996; Oberlin et al. 1996). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (Lapham et al. 1996). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (Feng et al. 1996). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (Feng et al. 1996; Endres et al. 1996; Brelot et al. 1997). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (Brelot et al. 1997; Lu et al. 1997).

Immunogen

Epitope: extracellular loop

Extracellular loop peptide of human CXCR4 (Berson et al. 1996; Leotscher et al. 1994).

Application

Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop detects level of C-X-C Chemokine Receptor 4 & has been published & validated for use in WB.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Western blot: 1:500 - 1000

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Purified IgG in PBS containing 0.02% sodium azide.

Storage and Stability

Can be refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable