400064
Anti-Glucose Transporter-4 Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-Glut-4
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SLC2A4(6517)
General description
Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.
Recognizes the ~40-43 kDa GLUT-4 protein.
Immunogen
Mouse
a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
Application
ELISA (0.5-1 µg/ml)
Frozen Sections (2-20 µg/ml)
Immunoblotting (1-10 µg/ml; see comments)
Immunoprecipitation (1-10 µg/100 µg tissue membrane)
Frozen Sections (2-20 µg/ml)
Immunoblotting (1-10 µg/ml; see comments)
Immunoprecipitation (1-10 µg/100 µg tissue membrane)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 0.1% BSA, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The Journal of physiology, 568(Pt 1), 229-242 (2005-08-06)
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Young Ju Lee et al.
Molecular medicine reports, 7(5), 1571-1578 (2013-04-03)
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