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Key Documents

343222

Sigma-Aldrich

Biotin, Fluorescein Conjugate

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.41

form

lyophilized solid

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

orange

solubility

DMF: 5 mg/mL
DMSO: 5 mg/mL
methanol: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

It can be used for the detection of avidin and streptavidin.

Application

EIA (1:1,000)

Tissue Staining (1:50 - 1:100)

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Other Notes

Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203
Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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