Saltar al contenido
Merck

Immunohistochemical recognition of human follicular dendritic cells (FDCs) in routinely processed paraffin sections.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2002-11-06)
Kunihiko Maeda, Mikio Matsuda, Hitoshi Suzuki, Hito-Aki Saitoh
RESUMEN

A number of monoclonal antibodies (MAbs) that recognize human follicular dendritic cells (FDCs) have been identified. Although some of them have already been applied individually in routine immunolabeling using formalin-fixed paraffin sections for diagnostic and experimental purposes, many antibodies are still employed only for immunolabeling using cryostat sections or particularly processed sections because they have been thought unsuitable for routine sections. A comprehensive examination re-evaluating their suitability in paraffin sections has not been reported. Accordingly, there is limited ability to examine the immunopathological contribution or diagnostic value of FDCs using routinely processed specimens or archived materials. In this study a broad panel of antibodies was systematically applied to the immunolabeling of paraffin sections of reactive tonsils or lymph nodes, in combination with advanced antigen retrieval (AR) techniques. Several antibodies, including Ki-M4p, X-11, 12B1, CNA.42, 1F8/BU32 (anti-CD21), BU38/1B12 (anti-CD23), Ber-MAC-DRC/To5 (anti-CD35), 1.4C3 (anti-CD106), NGFR5 (anti-nerve growth factor receptor p75), IIH6 (anti-CD55), 55K-2 (anti-fascin), and anti-S100 protein alpha-chain, were found to label FDCs in routine sections when combined with suitable AR techniques. Our results are easily adaptable for routine practice and provided useful suggestions concerning the immunopathological behavior and diversity of the particular cells.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
CD21 (EP3093) Rabbit Monoclonal Primary Antibody
Sigma-Aldrich
CD21 (2G9) Mouse Monoclonal Antibody
Sigma-Aldrich
CD35 (EP197) Rabbit Monoclonal Primary Antibody