Saltar al contenido
Merck

Carbamates: A study on genotoxic, cytotoxic, and apoptotic effects induced in Chinese hamster ovary (CHO-K1) cells.

Toxicology in vitro : an international journal published in association with BIBRA (2015-03-31)
Sonia Soloneski, Maciej Kujawski, Anna Scuto, Marcelo L Larramendy
RESUMEN

In vitro effects of the carbamates pirimicarb and zineb and their formulations Aficida® (50% pirimicarb) and Azzurro® (70% zineb), respectively, were evaluated in Chinese hamster ovary (CHO-K1) cells. Whereas the cytokinesis-blocked micronucleus cytome assay was employed to test for genotoxicity, MTT, neutral red (NR), and apoptosis evaluation were used as tests for estimating cell viability and succinic dehydrogenase activity, respectively. Concentrations tested were 10-300 μg/ml for pirimicarb and Aficida®, and 1-50 μg/ml for zineb and Azzurro®. All compounds were able to increase the frequency of micronuclei. A marked reduction in the nuclear division index was observed after treatment with 5 μg/ml of zineb and Azzurro® and 10 μg/ml of Azzurro®. Alterations in the cellular morphology not allowing the recognition of binucleated cells exposed to 300 μg/ml pirimicarb and Aficida® as well as 10-50 μg/ml zineb and Azzurro®. All four compounds induced inhibition of both cell viability and succinic dehydrogenase activity and trigger apoptosis in CHO-K1 cells, at least when exposed for 24 h. The data herein demonstrate the genotoxic and cytotoxic effects exerted by these carbamates and reveal the potential risk factor of these pesticides, still extensively used worldwide, for both human health and the environment.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Dimetilsulfóxido, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Dimetilsulfóxido, for molecular biology
Sigma-Aldrich
Dimetilsulfóxido, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Dimetilsulfóxido, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
Dimetilsulfóxido, anhydrous, ≥99.9%
Sigma-Aldrich
Yoduro de propidio, ≥94.0% (HPLC)
Sigma-Aldrich
Alcohol etílico puro, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
Dimetilsulfóxido, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Reagent Alcohol, reagent grade
Sigma-Aldrich
Alcohol etílico puro, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
Reagent Alcohol, 70%, used for histology tissue preparation
Sigma-Aldrich
Dimetilsulfóxido, PCR Reagent
Sigma-Aldrich
Reagent Alcohol, used for histology tissue preparation
Supelco
Ethanol solution, 50 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Sigma-Aldrich
Dimetilsulfóxido, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
Alcohol etílico puro, 190 proof, meets USP testing specifications
Sigma-Aldrich
Propidium iodide solution
Supelco
Ethanol solution, 100 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol solution, 10 mg/dL in H2O, pack of 10 × 1.2 mL ampules, certified reference material, Cerilliant®
Supelco
Ethanol solution, 80 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol solution, 500 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol solution, 300 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol solution, 10 % (v/v) in H2O, analytical standard
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.003% water
Sigma-Aldrich
Etanol, ≥99.5%, suitable for HPLC
Supelco
Ethanol solution, 200 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Sigma-Aldrich
Etanol, JIS special grade, 94.8-95.8%
Sigma-Aldrich
Etanol, ≥99.5%
Sigma-Aldrich
Reagent Alcohol, 95%, Used for histology tissue preparation
Supelco
Ethanol solution, 150 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®