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Merck

CompassLSM: axially swept light-sheet microscopy made simple.

Biomedical optics express (2021-11-09)
Yehe Liu, Andrew M Rollins, Michael W Jenkins
RESUMEN

Axially swept light-sheet microscopy (ASLM) is an effective method of generating a uniform light sheet across a large field of view (FOV). However, current ASLM designs are more complicated than conventional light-sheet systems, limiting their adaptation in less experienced labs. By eliminating difficult-to-align components and reducing the total number of components, we show that high-performance ASLM can be accomplished much simpler than existing designs, requiring less expertise and effort to construct, align, and operate. Despite the high simplicity, our design achieved 3.5-µm uniform optical sectioning across a >6-mm FOV, surpassing existing light-sheet designs with similar optical sectioning. With well-corrected chromatic aberration, multi-channel fluorescence imaging can be performed without realignment. This manuscript provides a comprehensive tutorial on building the system and demonstrates the imaging performance with optically cleared whole-mount tissue samples.

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Sigma-Aldrich
Anticuerpo anti-NeuN, clon A60, conjugado con Alexa Fluor 555, clone A60, from mouse, ALEXA FLUOR 555