Saltar al contenido
Merck

TrkC Is Essential for Nephron Function and Trans-Activates Igf1R Signaling.

Journal of the American Society of Nephrology : JASN (2021-01-01)
Carolin Lepa, Sascha Hoppe, Antje Stöber, Boris V Skryabin, Laura Katharina Sievers, Barbara Heitplatz, Giuliano Ciarimboli, Ute Neugebauer, Maja T Lindenmeyer, Clemens D Cohen, Hannes C A Drexler, Peter Boor, Thomas Weide, Hermann Pavenstädt, Britta George
RESUMEN

Injury to kidney podocytes often results in chronic glomerular disease and consecutive nephron malfunction. For most glomerular diseases, targeted therapies are lacking. Thus, it is important to identify novel signaling pathways contributing to glomerular disease. Neurotrophic tyrosine kinase receptor 3 (TrkC) is expressed in podocytes and the protein transmits signals to the podocyte actin cytoskeleton. Nephron-specific TrkC knockout (TrkC-KO) and nephron-specific TrkC-overexpressing (TrkC-OE) mice were generated to dissect the role of TrkC in nephron development and maintenance. Both TrkC-KO and TrkC-OE mice exhibited enlarged glomeruli, mesangial proliferation, basement membrane thickening, albuminuria, podocyte loss, and aspects of FSGS during aging. Igf1 receptor (Igf1R)-associated gene expression was dysregulated in TrkC-KO mouse glomeruli. Phosphoproteins associated with insulin, erb-b2 receptor tyrosine kinase (Erbb), and Toll-like receptor signaling were enriched in lysates of podocytes treated with the TrkC ligand neurotrophin-3 (Nt-3). Activation of TrkC by Nt-3 resulted in phosphorylation of the Igf1R on activating tyrosine residues in podocytes. Igf1R phosphorylation was increased in TrkC-OE mouse kidneys while it was decreased in TrkC-KO kidneys. Furthermore, TrkC expression was elevated in glomerular tissue of patients with diabetic kidney disease compared with control glomerular tissue. Our results show that TrkC is essential for maintaining glomerular integrity. Furthermore, TrkC modulates Igf-related signaling in podocytes.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Insulina solution human, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
Anti-β-Tubulin antibody, Mouse monoclonal, ~2.0 mg/mL, clone AA2, purified from hybridoma cell culture
Sigma-Aldrich
Transcreener® GDP TR-FRET Assay