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Merck

Protocol for brain-wide or region-specific microglia depletion and repopulation in adult mice.

STAR protocols (2020-12-31)
Emily F Willis, Jana Vukovic
RESUMEN

The advent of tools enabling the direct manipulation of microglia has furthered our understanding of their role in health and disease. Here, we present a detailed protocol allowing for microglia turnover in adult CX3CR1 creERT2 × iDTR or CX3CR1 creERT2 × iDTR × tdTomatoflox mice, either in a brain-wide or region-specific manner, and their subsequent isolation for downstream applications. This protocol may be used to explore microglia biology and their putative region-specific heterogeneous functional diversity, expanding our understanding of their importance in various neuroinflammatory conditions. For complete details on the use and execution of this protocol, please refer to Willis et al. (2020).

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Roche
ADNasa I, grade II, from bovine pancreas
Sigma-Aldrich
Tamoxifeno, ≥99%
Supelco
Acrylamide:N,N′-Methylenebisacrylamide 37.5:1 solution, suitable for electrophoresis, mixture of acrylamide and BIS 37.5:1, 40% in H2O