Skip to Content
Merck
  • Aluminium induced endoplasmic reticulum stress mediated cell death in SH-SY5Y neuroblastoma cell line is independent of p53.

Aluminium induced endoplasmic reticulum stress mediated cell death in SH-SY5Y neuroblastoma cell line is independent of p53.

PloS one (2014-06-01)
Syed Husain Mustafa Rizvi, Arshiya Parveen, Anoop K Verma, Iqbal Ahmad, Md Arshad, Abbas Ali Mahdi
ABSTRACT

Aluminium (Al) is the third most abundant element in the earth's crust and its compounds are used in the form of house hold utensils, medicines and in antiperspirant etc. Increasing number of evidences suggest the involvement of Al+3 ions in a variety of neurodegenerative disorders including Alzheimer's disease. Here, we have attempted to investigate the role of Al in endoplasmic reticulum stress and the regulation of p53 during neuronal apoptosis using neuroblastoma cell line. We observed that Al caused oxidative stress by increasing ROS production and intracellular calcium levels together with depletion of intracellular GSH levels. We also studied modulation of key pro- and anti-apoptotic proteins and found significant alterations in the levels of Nrf2, NQO1, pAKT, p21, Bax, Bcl2, Aβ1-40 and Cyt c together with increase in endoplasmic reticulum (ER) stress related proteins like CHOP and caspase 12. However, with respect to the role of p53, we observed downregulation of its transcript as well as protein levels while analysis of its ubiquitination status revealed no significant changes. Not only did Al increase the activities of caspase 9, caspase 12 and caspase 3, but, by the use of peptide inhibitors of specific and pan-caspases, we observed significant protection against neuronal cell death upon inhibition of caspase 12, demonstrating the prominent role of endoplasmic reticulum stress generated responses in Al toxicity. Overall our findings suggest that Al induces ER stress and ROS generation which compromises the antioxidant defenses of neuronal cells thereby promoting neuronal apoptosis in p53 independent pathway.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, JIS special grade, ≥99.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide solution, 50 wt. % in H2O
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.0%, suitable for absorption spectrum analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium bicarbonate-12C, 99.9 atom % 12C
Pricing and availability is not currently available.
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)
Pricing and availability is not currently available.
Supelco
Dimethyl sulfoxide, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
Pricing and availability is not currently available.
Sigma-Aldrich
Aluminum chloride hexahydrate, meets EP, BP, USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Z-Leu-Leu-Leu-al, ≥90% (HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
Pricing and availability is not currently available.
Supelco
Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, SAJ first grade, ≥99.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
Pricing and availability is not currently available.
USP
Dimethyl sulfoxide, United States Pharmacopeia (USP) Reference Standard
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC, ≥99.7%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, ACS reagent, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Aluminum chloride hexahydrate, ReagentPlus®, 99%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Pricing and availability is not currently available.
Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, Vetec, reagent grade, 99%
Pricing and availability is not currently available.