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G006134

HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)
column: Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)
mobile phase: (A) 20 mM sodium phosphate buffer, pH 6.0, (B) A + 1.0 M NaCl
gradient: 0-25 min from 0-75% B, 15 min prewash
flow rate: 0.75 mL/min
column temp.: Ambient
detector: 214 nm
injection: 5 μL
sample: each 1 mg/mL

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

Legal Information

Proteomix is a registered trademark of Sepax Technologies

Analyte

Description
    Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
    Cytochrome c from equine heart, ≥95% (SDS-PAGE)
    Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein

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