Product P0065 (Phospholipase D) is sold by activity units per milliliter (units/mL). The protein amount is not measured; only the units/mL are provided for this product.
P0065
Phospholipase D from Streptomyces chromofuscus
≥50,000 units/mL, buffered aqueous glycerol solution
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Pricing and availability is not currently available.
Recommended Products
form
buffered aqueous glycerol solution
Quality Level
specific activity
≥50,000 units/mL
mol wt
~60 kDa
shipped in
dry ice
storage temp.
−70°C
Looking for similar products? Visit Product Comparison Guide
General description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.[1]
Application
Biochem/physiol Actions
Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline.[4] PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway.[5] PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.[6]
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Unit Definition
One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.
Physical form
Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Phospholipase D: a downstream effector of ARF in granulocytes.
Cockcroft S
Science, 263(5146), 523-526 (1994)
Lecithin: a by-product of biodiesel production and a source of choline for dairy cows
Italian Journal of Animal Science, 11:2 (2012)
Dynamics and function of phospholipase D and phosphatidic acid during phagocytosis.
Traffic, 7(3), 365-377 (2006)
Eliminating office barriers to the handicapped patient.
Henson JL
The New dentist, 9(6), 30-32 (1979)
Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose.
Imamura S and Horiuti Y
Journal of Biochemistry, 85(1), 79-95 (1979)
-
How can I estimate the amount of protein in micrograms (μg) for P0065?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
