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MAK367

Sigma-Aldrich

Nitrite Assay Kit (Griess Reagent)

sufficient for 200 colorimetric tests

Synonym(s):

Nitrite Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

relevant disease(s)

immunological diseases

storage temp.

2-8°C

General description

Nitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and can also be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxiadependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration.

Application


  • Convenient environmentally friendly on-site quantitative analysis of nitrite and nitrate in seawater based on polymeric test kits and smartphone application.: This research develops an eco-friendly and portable method for nitrite and nitrate analysis in seawater, employing polymeric test kits and a smartphone application for data analysis, facilitated by the Griess reagent for nitrite detection. (Choodum et al., 2020).

  • MiR-124 inhibits spinal neuronal apoptosis through binding to GCH1.: The study investigates the role of miR-124 in preventing spinal neuronal apoptosis, utilizing a nitrite assay kit to measure nitric oxide levels as part of the biochemical analysis. (Yuan et al., 2019).

  • Humoral factors in ALS patients during disease progression.: This paper examines the changes in humoral factors in ALS patients, measuring nitric oxide levels using a nitrite assay kit to assess inflammation and oxidative stress. (Ehrhart et al., 2015).

  • Inducible nitric oxide synthase-nitric oxide plays an important role in acute and severe hypoxic injury to pancreatic beta cells.: The research highlights the significance of nitric oxide in hypoxic injury to pancreatic beta cells, using a nitrite assay kit to quantify nitric oxide levels. (Ko et al., 2008).

Suitability

The kit is suitable for the detection of Nitric Oxide in animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma and urine.

Principle

The Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at A540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.

replaced by

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8K19K05440
8G25K05440
8B14K05440
7F30K05440
7K22K05440

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Eloi Franco-Trepat et al.
British journal of pharmacology, 179(2), 270-286 (2021-10-14)
Osteoarthritis, a major cause of disability in developed countries does not have effective treatment. Activation of TLR4 and innate immune response factors contribute to osteoarthritis progressive cartilage degradation. There are no clinically available TLR4 inhibitors. Interestingly, the antidepressant amitriptyline could
Claudia Reyes-Goya et al.
The British journal of nutrition, 1-14 (2022-01-11)
Despite numerous reports on the beneficial effects of olive oil in the cardiovascular context, very little is known about the olive tree's wild counterpart (Olea europaea, L. var. sylvestris), commonly known as acebuche (ACE) in Spain. The aim of this

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can this kit be used to examine nitric oxide release from polymer fibers?

    1 answer

    1. The kit is suitable for samples such as animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma, and urine. No specific experiments have been performed for the analysis in a polymer fiber sample.

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