The reagents used with the MAK311-1KT Peroxide Assay Kit create an acidic environment in the well plate, and Phenol Red should not have measurable absorbance at the kit's wavelength of 585 nm.
As an extra precaution, it is recommended to dilute the H2O2 standard in the same media that is used on the cells to account for any background or chemical interactions from the media. Furthermore, it is best for samples to contain less than 1 mM pyruvate.
MAK311
Peroxide Assay Kit
sufficient for 250 colorimetric tests
Synonym(s):
Hydrogen Peroxide Test Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
detection method
colorimetric
relevant disease(s)
cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders
storage temp.
2-8°C
Related Categories
General description
Peroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Application
Peroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates.[1]
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium.
Principle
The Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe3+ −xylenol orange reaction, in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 μM H2O2 equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Storage Class Code
8B - Non-combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Is it possible to use the MAK311-1KT Peroxide Assay Kit to measure peroxide in lysed cells that are grown in Minimum Essential Medium Eagle containing Phenol Red at a concentration of 0.011 g/liter?
1 answer-
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Is 1% TWEEN in the sample compatible with the MAK311 peroxide assay kit?
1 answer-
The tween in samples should remain below 0.25%. If the sample contains 1% tween it is recommend diluting 4x to 0.25%, assuming the sample has enough peroxide to still be in the detectable range. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format.
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