SNL 76/7 Cell Line from mouse
7032801, mouse esophagus, Fibroblast
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About This Item
UNSPSC Code:
41106514
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product name
SNL 76/7 Cell Line from mouse, 07032801
biological source
mouse esophagus
description
Mouse SIM strain embryonic fibroblast
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse SIM strain embryonic fibroblast
Cell Line Description
SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.
Culture Medium
Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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