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82526

Sigma-Aldrich

Protein A soluble from Staphylococcus aureus (Cowan strain)

powder, white

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

Staphylococcus aureus

Quality Level

conjugate

unconjugated

form

powder

species reactivity

mouse, cat, monkey, pig, dog, human, rabbit, guinea pig

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

color

white

capacity

~10 mg/mg binding capacity (human IgG)

solubility

H2O: 1 mg/mL, clear, colorless

UniProt accession no.

storage temp.

2-8°C

Gene Information

Staphylococcus aureus subsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448)

General description

Protein A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. This protein is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel alpha-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also associates with IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat.
Protein A is involved in regulating anti-tumor, toxic, and carcinogenic functions. In addition to acting as an immunomodulator, it also has antifungal and antiparasitic properties.

Application

Protein A may be conjugated with various reporter molecules such as fluorescent dyes (FITC), enzyme markers (peroxidase, beta-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates may be used to detect immunoglobulins using immunochemical assays such as western blotting, immunohistochemistry, and ELISA. In addition, protein A may also be immobilized on a solid supports like agarose or acrylic beads for the purification of polyclonal or monoclonal immunoglobulins. It is also routinely used for immunoprecipitation assays. Furthermore, protein A has been used for affinity chromatography and SDS-PAGE assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biswarup Basu et al.
The Journal of biological chemistry, 285(35), 27026-27032 (2010-07-02)
Dopamine is a catecholamine neurotransmitter, which plays an important role in the regulation of T cell functions. In activated T cells from normal volunteers, stimulation of D(1) and D(2) dopamine receptors inhibit cell proliferation and cytokine secretion. However, there is
J I Flock et al.
The EMBO journal, 6(8), 2351-2357 (1987-08-01)
The gene encoding the fibronectin-binding protein (FNBP) from Staphylococcus aureus strain 8325-4 was isolated from a gene bank in pBR322. The original clone, containing a 6.5-kb insert, gave a functional product present in the periplasm of Escherichia coli. Analysis of
Laura Gámez-Díaz et al.
Frontiers in immunology, 9, 720-720 (2018-05-10)
The diagnosis of lipopolysaccharide-responsive beige-like-anchor-protein (LRBA) deficiency currently relies on gene sequencing approaches that do not support a timely diagnosis and clinical management. We developed a rapid and sensitive test for clinical implementation based on the detection of LRBA protein

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