Yes. When performing fluorescent staining procedures, aldehydes and alcohols are common fixatives. Paraformaldehyde solutions are prepared without the use of methanol. It is believed that methanol can adversely affect the staining of membrane bound proteins. It it for this reason that freshly prepared paraformaldehyde solutions are preferred over formaldehyde solutions that may contain up to 10 or 15% methanol to keep the formaldehyde from re-polymerizing back into solid paraformaldehyde. When using paraformaldehyde to fix tissue, frozen sections are preferred over paraffin processing.
42874
Tetramethylrhodamine isothiocyanate–Dextran
average mol wt 40,000
Synonym(s):
TRITC-Dextran 40, TRITC-Dextran
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About This Item
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biological source
synthetic
Quality Level
form
powder
mol wt
average mol wt 40,000
extent of labeling
0.001-0.008 mol/mol TRITC glucose
fluorescence
λex 550 nm; λem 577 nm±5 nm in 0.1 M Tris pH 8.0
application(s)
cell analysis
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General description
Tetramethylrhodamine isothiocyanate–Dextran, also known as TRITC-Dextran or TRITC-Dextran 40, is a fluorescent dye and a novel drug. The fluorescent activity of TRITC-Dextran is detected at 555nm wavelength.[1] The TRITC-Dextran molecules are released in the cells through passive diffusion due to a concentration gradient.[2]
Application
Tetramethylrhodamine isothiocyanate–Dextran (TRITC-Dextran 40) is used as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular drug delivery.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Can I use paraformaldehyde to fix the tissue and then use fluorescence imaging? For example: Inject Tritc i.v. and then extract the brain tissue. The brain would be fixed in paraformaldehyde to be sliced later and imaged for fluorescence
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