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A92775

Sigma-Aldrich

Arsenazo III

calcium-sensitive dye, Powder

Synonym(s):

2,2′-(1,8-Dihydroxy-3,6-disulfonaphthylene-2,7-bisazo)bisbenzenearsonic acid, 2,7-Bis(2-arsonophenylazo)chromotropic acid

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About This Item

Linear Formula:
(HO)2C10H2(SO3H)2(N=NC6H4AsO3H2)2
CAS Number:
Molecular Weight:
776.37
Beilstein:
5717957
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Arsenazo III, calcium-sensitive dye

Quality Level

form

powder

technique(s)

titration: suitable

impurities

<0.1% Ca

color

brown to very dark brown, and Black

mp

>320 °C (lit.)

solubility

ammonium hydroxide: 1 mg/mL

λmax

530 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(\N=N\c2ccccc2[As](O)(O)=O)c(cc3cc(c(\N=N\c4ccccc4[As](O)(O)=O)c(O)c13)S(O)(=O)=O)S(O)(=O)=O

InChI

1S/C22H18As2N4O14S2/c29-21-18-11(9-16(43(37,38)39)19(21)27-25-14-7-3-1-5-12(14)23(31,32)33)10-17(44(40,41)42)20(22(18)30)28-26-15-8-4-2-6-13(15)24(34,35)36/h1-10,29-30H,(H2,31,32,33)(H2,34,35,36)(H,37,38,39)(H,40,41,42)/b27-25+,28-26+

InChI key

UQHVTNUJRKELCE-NBHCHVEOSA-N

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Application

Arsenazo III has been used as a color forming reagent to determine uranium concentration.

Biochem/physiol Actions

Arsenazo III is a metallochromic dye. Arsenazo III is used in the determination of calcium in biological samples. It is used to evaluate calcium transport in permeabilized cells. It is also used for the detection of rare earth metals (polyvalent metal ions). Arsenazo III is used for the spectrophotometric determination of uranium and thorium.

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Analytica Chimica Acta, 68, 73-73 (1974)
Carmen Hogendoorn et al.
Applied and environmental microbiology, 84(8) (2018-02-18)
Recently, methanotrophic and methylotrophic bacteria were found to utilize rare earth elements (REEs). To monitor the REE content in culture media of these bacteria, we have developed a rapid screening method using the Arsenazo III (AS III) dye for spectrophotometric
Mohammad Hassan Omidi et al.
Journal of colloid and interface science, 504, 68-77 (2017-05-23)
The present study devoted to description of efficient removal of radioactive UO
J W Janssen et al.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies, 29(3), 197-201 (1991-03-01)
The widely used calcium dye reagent, o-cresolphthalein complexone, was replaced by the less well known metallochromic indicator Arsenazo III in a selective multitest analyser. Calibration stability increased from 12 hours to more than 48 hours. With the Arsenazo III reagent
R Kuroda et al.
Talanta, 37(6), 619-624 (1990-06-01)
A derivative spectrophotometric method has been developed for the simultaneous determination of microgram quantities of uranium and thorium with Arsenazo III in hydrochloric acid medium. The second-derivative absorbances of the uranium and thorium Arsenazo III complexes at 679.5 and 684.4

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