11363905910

Digoxigenin-11-ddUTP

solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)

• Synonym(s): digoxigenin
• UNSPSC Code: 41116100

Properties

• Quality Level: 100
• Assay: 85% (HPLC)
• form: solution
• mol wt: (Molecular weight: M_r = 1074.7 (DIG-11-ddUTP-Li₄))
• packaging: pkg of 25 μL (25 nmol; 1mM)
• manufacturer/tradename: Roche
• storage temp.: −20°C

Description

General description

Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling. Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.

Application

Digoxigenin-11-ddUTP has been used:

• for labeling of DNA fragments in linker histone binding assay
• for end labeling of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay and labelling of ATP citrate lyase gene for gel shift assay

for end labeling of DNA for fragmentation reactions

Quality

Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.

Formula: C₄₃H₆₁N₄O₂₀P3Li₄

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash