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121800-M

Sigma-Aldrich

Advanced Glycation Endproduct-BSA

AGE-BSA has been reported to induce apoptosis in cultured human umbilical vein endothelial cells and inhibit nitric oxide synthase activity in proximal tubular epithelial cells.

Synonym(s):

AGE-BSA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.25
Pricing and availability is not currently available.

Quality Level

100

Assay

≥95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

storage temp.

−70°C

Related Categories

General description

Prepared by reacting BSA with glycoaldehyde under sterile conditions. Glycated-BSA shows a 5,000 - 10,000% increase in fluorescence as compared to normal BSA (confirmed by fluorescence spectrophotometry, excitation/emission 370/440 nm). AGE-BSA has been reported to induce apoptosis in cultured human umbilical vein endothelial cells and inhibit nitric oxide synthase activity in proximal tubular epithelial cells. Advanced glycation end products and their receptors have been implicated in the pathogenesis of diabetes, induction of proinflammatory cytokines, and stimulation of smooth muscle proliferation, and fibronectin production.
Prepared by reacting bovine serum albumin (BSA) with glycoaldehyde under sterile conditions. Fluorescence of AGE-BSA is confirmed by fluorescence spectrophotometry, excitation/emission = 370/440 nm. Glycated BSA shows a 5000-10,000% increase in fluorescence compared to control BSA. AGE and their receptors have been implicated in the pathogenesis of diabetes, induction of pro-inflammatory cytokines, and stimulation of smooth muscle proliferation and fibronectin production. AGE-BSA has also been shown to induce apoptosis in cultured human umbilical vein endothelial cells (HUVEC) and inhibit nitric oxide synthase activity in proximal tubular epithelial cells of the kidney.

Warning

Toxicity: Standard Handling (A)

Physical form

In sterile-filtered PBS.

Other Notes

Okamoto, T., et al. 2002. Microvasc. Res.63, 186.
Ohgami, N., et al. 2001. J. Biol. Chem.276, 3195.
Wang, R., et al. 2001. J. Nippon Med. Sch.68, 472.
Sakata, N., et al. 2000. J. Atheroscler. Thromb.7, 169.
Verbeke, P., et al. 2000. Biochim. Biophys. Acta1502, 481.
Farboud, B., et al. 1999. Mol. Vis.5, 11.
Huang, J.-S., et al. 1999. Biochem. J.342, 231.
Min, C., et al. 1999. Diabetes Res. Clin. Pract.46, 197.
Neumann, A., et al. 1999. FEBS Lett.453, 283.
Stitt, A.W., et al. 1999. Biochem. Biophys. Res. Commun.256, 549.
Nazaimoon, W. and Bak, K. 1998. Malays. J. Pathol.20, 83.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–4 of 4 Questions  

  1. What is the molecular weight of AGE-BSA, 121800-M?

    1 answer

    1. The predicted molecular weight of AGE-BSA is approximately 66.5 kDa.

      Helpful?

  2. What is the concentration of the product supplied? 121800-10MG

    1 answer

    1. The concentration of the item is 10 mg/mL. This value is reported in the lot-specific Certificate of Analysis.

      Helpful?

  3. If the diluted AGE-RAGE is prepared a year ago and was stored in -60 degree, can it still be used?

    1 answer

    1. Unfortunately, the solution stability has not been determined. However, other sources state that following reconstitution, this chemical is stable at -20 to -70 °C for only 3 months.

      Helpful?

  4. What is the molecular weight of the product 121800-M (AGE-BSA)? and what is the degree of glycation?

    1 answer

    1. AGE-BSA has a predicted MW of ~66.5 kDa. The degree of glycation is not determined.

      Helpful?

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