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07-455

Sigma-Aldrich

Anti-CoREST Antibody

Upstate®, from rabbit

Synonym(s):

Anti-COREST, Anti-RCOR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RCOR1(23186)

General description

The CoREST gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.

Specificity

Recognizes CoREST.

Immunogen

GST fusion protein corresponding to residues 109-293 of human CoREST.

Application

Detect CoREST with Anti-CoREST Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ChIP, ICC, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Quality

routinely evaluated by immunoblot in RIPA lysates from A431, 3T3/A31, Jurkat,and Hela cells

Target description

66 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, and 0.05% sodium azide as a preservative.
Protein A purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of immunogenic MHC class II Tyrosinase-derived peptides using HLA-DR1 and HLA-DR4 transgenic mice.
R B V Horton,S A S Laversin,S P Reeder,R C Rees,S E B McArdle
Protein and peptide letters null
ZNF198 stabilizes the LSD1-CoREST-HDAC1 complex on chromatin through its MYM-type zinc fingers.
Gocke, CB; Yu, H
Testing null
Benoît Laurent et al.
Journal of cell science, 125(Pt 4), 993-1002 (2012-03-09)
Gfi-1B is a transcriptional repressor essential for the regulation of erythropoiesis and megakaryopoiesis. Here we identify Gfi-1B p32, a Gfi-1B isoform, as essential for erythroid differentiation. Gfi-1B p32 is generated by alternative splicing and lacks the two first zinc finger
Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST.
Dharap, A; Pokrzywa, C; Vemuganti, R
ASN Neuro null
Allana Schooley et al.
Journal of cell science, 128(18), 3466-3477 (2015-08-01)
The metazoan nucleus breaks down and reassembles during each cell division. Upon mitotic exit, the successful reestablishment of an interphase nucleus requires the coordinated reorganization of chromatin and formation of a functional nuclear envelope. Here, we report that the histone

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