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ZRB1944

Sigma-Aldrich

Anti-TIMP-1 Antibody, clone 1H19 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

Metalloproteinase inhibitor 1;Erythroid-potentiating activity;EPA;Fibroblast collagenase inhibitor;Collagenase inhibitor;Tissue inhibitor of metalloproteinases 1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

1H19, recombinant monoclonal

Description

1H19 Clone

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 23.17 kDa
observed mol wt ~31 kDa

Produit purifié par

using Protein A

Espèces réactives

human

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG

Séquence de l'épitope

N-terminal half

Numéro d'accès Protein ID

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TIMP1(7076)

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1H19 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects TIMP-1. It targets an epitope within 15 amino acids from the N-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human TIMP-1.

Application

Quality Control Testing

Evaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TIMP-1 in MDA-MB-231 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TIMP-1 in HeLa cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound TIMP1 peptide with a KD of 1.2 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TIMP-1 in human prostate tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in MDA-MB-231 cell lysate

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TIMP-1 in MDA-MB-231 cell lysate.

Description de la cible

Metalloproteinase inhibitor 1 (UniProt: P01033; also known as Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, TIMP-1) is encoded by the TIMP1 (also known as CLGI, TIMP) gene (Gene ID: 7076) in human. The tissue inhibitors of metalloproteinases (TIMPs) represent a family of ubiquitous proteins which are natural inhibitors of the matrix metalloproteinases (MMPs). There are four known members of the TIMP family that are designated as TIMP-1, 2, 3, and 4. TIMPs are released during tissue damage to facilitate tissue remodeling through degradation and reorganization of the extracellular matrix. During this process MMPs are involved in inflammatory response through proteolytic maturation of cytokines. These activities are regulated through a 1:1 stoichiometric interaction of MMPs with TIMPs and these interactions are tightly controlled. TIMP-1 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein (aa 24-207). It is the best characterized inhibitor of MMP activity and regulates 14 of the 24 known MMPs. It inhibits MMP activity through the binding of its N-terminus with the targeted MMP, which results in chelation of Zn2+ from the enzyme active site. TIMP-1 levels are elevated in plasma of cancer subjects and its higher levels are associated with worse clinical outcomes. Stroma of human prostate and colon cancer is reported to express higher levels of TIMP-1 compared to their normal counterparts. This increased expression is reported to promotes tumor growth. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Knight, BE., et al. (2019). Front. Mol. Neurosci. 12; 220; Gong, Y., et al. (2013). PLoS ONE 8(10): e77366).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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