Accéder au contenu
Merck
Toutes les photos(9)

Key Documents

ZRB1432

Sigma-Aldrich

Anti-p-Myosin LC 2 (Ser19) Antibody, clone 2C10 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

Myosin regulatory light polypeptide 9

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

2C10, recombinant monoclonal

Description

recombinant, expressed in HEK 293 cells

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 19.83 kDa
observed mol wt ~19 kDa

Produit purifié par

using Protein A

Espèces réactives

mouse, rat, human

Réactivité de l'espèce (prédite par homologie)

bovine, feline, porcine, monkey, canine

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotype

IgG

Séquence de l'épitope

Unknown

Numéro d'accès Protein ID

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MYL9(10398)

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 2C10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects myosin light chain 2 phosphorylated on serine 19.

Immunogène

KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 19 from the N-terminal region of human Myosin Light Chain 2 (MLC2).

Application

Quality Control Testing

Evaluated by Western Blotting in lysates from C2C12 cells.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected phospho-Myosin light chain 2 (Ser19) in lysate from C2C12 cells.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-Myosin LC 2 (Ser19) in C6 and HEK293 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-Myosin LC 2 (Ser19) in human uterus tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound phospho-Myosin Light Chain 2 (Ser-19) with a KD of 6.0 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Myosin regulatory light polypeptide 9 (UniProt: P24844; also known as 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2 smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1) is encoded by the MLY9 (also known as MLC2, MRLC1, MYRL2) gene (Gene ID: 10398) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). MLC-2C plays an important role in regulation of both smooth muscle and non-muscle cell contractile activity via its phosphorylation. It is phosphorylated at threonine 18 and serine 19 by myosin light chain kinase (MLCK) in a Ca2+/CaM-dependent manner. Rho kinase (ROCK) is also known to phosphorylates MLC-2C at serine 19. Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells. Phosphorylation is also required for myotube formation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kumar, CC., et al. (1989). Biochemistry. 28(9); 4027-4035).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique