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W1754

Sigma-Aldrich

Eau

PCR Reagent, suitable for PCR

Synonyme(s) :

H2O

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About This Item

Formule linéaire :
H2O
Numéro CAS:
7732-18-5
Poids moléculaire :
18.02
Numéro Beilstein :
2050024
Numéro CE :
231-791-2
Numéro MDL:
MFCD00011332
Code UNSPSC :
12191602
ID de substance PubChem :
24900779
Nomenclature NACRES :
NA.25

product name

Eau, PCR Reagent

Qualité

PCR Reagent

Densité de vapeur

<1 (vs air)

Pression de vapeur

3 mmHg

Stérilité

sterile-filtered

Forme

liquid

Conditionnement

vial of 1.5 mL

Technique(s)

PCR: suitable

Indice de réfraction

n20/D 1.34 (lit.)

pH

5-7

Point d'ébullition

100 °C (lit.)

Pf

0 °C (lit.)

Densité

1.000 g/mL at 3.98 °C (lit.)

Activité étrangère

DNase, none detected
RNase, none detected

Chaîne SMILES 

O

InChI

1S/H2O/h1H2

Clé InChI

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Catégories apparentées

Description générale

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Adéquation

Suitable for polymerase chain reaction (PCR)

Autres remarques

Easily compare specifications for Water products with the Water specification table.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Journal of Applied Microbiology, 111(2), 484-490 (2011)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
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Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
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