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T8575

Sigma-Aldrich

Anti-Tryptophan Hydroxylase antibody produced in sheep

affinity isolated antibody, buffered aqueous glycerol solution

Synonyme(s) :

Anti-TPH

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About This Item

Numéro MDL:
MFCD00282500
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

sheep

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous glycerol solution

Utilisation

sufficient for 10 blots

Espèces réactives

mammals, human

Technique(s)

western blot: 1:1,000 using human dorsal raphe nucleus

Numéro d'accès UniProt

P17752

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... TPH1(7166)
mouse ... Tph1(21990)
rat ... Tph1(24848)

Catégories apparentées

Description générale

The tryptophan hydroxylase 1 (TPH1) gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. TPH1, is a 444 amino acid protein expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.
Tryptophan Hydroxylase (TPH) is a member of the aromatic amino acid hydroxylases (AAAH) family.

Immunogène

recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Application

Anti-Tryptophan Hydroxylase antibody produced in sheep has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence
  • immunoperoxidase staining

Actions biochimiques/physiologiques

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Forme physique

Solution in 150 mM NaCl, 10 mM HEPES, pH 7.5, 100 μg per mL BSA, and 50% glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

R C Drugan et al.
Neuroscience, 253, 221-234 (2013-09-04)
Prior adverse experience alters behavioral responses to subsequent stressors. For example, exposure to a brief swim increases immobility in a subsequent swim test 24h later. In order to determine if qualitative differences (e.g. 19°C versus 25°C) in an initial stressor
Tryptophan hydroxylase 1 gene polymorphisms alter prefrontal cortex activation during response inhibition.
Ruocco AC
Neuropsychology, 30, 18-27 (2016)
Matthew W Hale et al.
Journal of chemical neuroanatomy, 41(4), 219-226 (2011-06-07)
Tryptophan hydroxylase is the rate-limiting enzyme in the biosynthesis of serotonin (5-hydroxytryptamine; 5-HT). Two isoforms of tryptophan hydroxylase, derived from different genes, tph1 and tph2, have been identified. The tph1 isoform is expressed in peripheral tissues, whereas tph2 is brain
Rachel A Walker et al.
The European journal of neuroscience, 51(3), 866-880 (2019-08-04)
Aversive, positive prediction error (+PE) provides a mechanism to update and increase future fear to uncertain threat predictors. The ventrolateral periaqueductal grey (vlPAG) has been offered as a neural locus for +PE computation. Yet, a causal demonstration of vlPAG +PE
Kristina M Wright et al.
eLife, 8 (2019-03-08)
Faced with potential harm, individuals must estimate the probability of threat and initiate an appropriate fear response. In the prevailing view, threat probability estimates are relayed to the ventrolateral periaqueductal gray (vlPAG) to organize fear output. A straightforward prediction is
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