This product is stable at room temperature at least until the assigned retest date reported in the Certificate of Analysis. Products that, while considered stable, may be subject to degradation over time are assigned a periodic retest date to confirm quality. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/t2569#product-documentation
T2569
Trizma® hydrochloride solution
pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture
Synonyme(s) :
Tris hydrochloride solution
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About This Item
Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Qualité
BioPerformance Certified
for molecular biology
Niveau de qualité
Forme
solution
Concentration
1 M
Technique(s)
cell culture | mammalian: suitable
Impuretés
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
pH
7.8
Plage de pH utile
7.0-9.0
Absorption
≤0.05 at 290 at 40%
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Description générale
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0.[1] It displays negligible binding towards metal in solutions.[2] Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies.[3] Its low ionic mobility finds usage in capillary electrochromatography as well.[3]
Application
Trizma® hydrochloride solution has been used:
- as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells[4]
- in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer[5]
- in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye[6]
Informations légales
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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