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SRP3050

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Synonyme(s) :

CSF-2, MGI-1GM, pluripoietin-a

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Pureté

≥98% (SDS-PAGE)

Forme

lyophilized

Puissance

≤0.1 ng/mL

Poids mol.

14.6 kDa

Conditionnement

pkg of 20 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1 EU/μg endotoxin, tested

Couleur

white to off-white

Numéro d'accès UniProt

P04141

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... CSF2(1437)

Catégories apparentées

Description générale

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. Recombinant human GM-CSF is a 14.6kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

Application

Granulocyte-Macrophage Colony-Stimulating Factor human has been used in the medium for plating of the cells. It has been used in culture medium for the differentiation of human peripheral blood mononuclear cells.

Actions biochimiques/physiologiques

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration and enhances the functional activity of the mature end-cells.

Séquence

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Forme physique

Lyophilized from 10 mM Sodium Citrate, pH 3.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Siddappa N Byrareddy et al.
PloS one, 10(10), e0140689-e0140689 (2015-10-16)
Lectin-like molecules and their receptors are cell surface molecules that have been shown to play a role in either facilitating infection or serving as transporters of HIV/SIV in vivo. The role of these lectin-like molecules in the pathogenesis of HIV/SIV
Candida parapsilosis produces prostaglandins from exogenous arachidonic acid and OLE2 is not required for their synthesis.
Grozer Z, et al.
Virulence, 6, 85-92 (2015)
Association of genetic variations in the CSF2 and CSF3 genes with lung function in smoking-induced COPD.
He JQ
The European Respiratory Journal, 32, 25-34 (2008)
Growth of clonogenic myeloblastic leukemic cells in the presence of human recombinant erythropoietin in addition to various human recombinant hematopoietic growth factors.
Asano Y
Blood, 72, 1682-1686 (1988)
Rapamycin inhibits GM-CSF-induced neutrophil migration.
Gomez-Cambronero J
Febs Letters, 550, 94-100 (2003)
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