SAB4200809

Anti-Epidermal Growth Factor (EGF) Receptor, Mouse monoclonal

clone 29.1, purified from hybridoma cell culture

• Synonyme(s) : EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: 29.1, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: ~170 kDa
• Espèces réactives: human, bovine, mouse
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~1 mg/mL
• Technique(s): immunoblotting: 0.5-1 μg/mL using human epidermoid carcinoma A431 cell line extract.; immunofluorescence: 2-4 μg/mL using human epidermoid carcinoma A431 cells.
• Isotype: IgG1
• Numéro d'accès UniProt: P00533
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... EGFR(1956)

Description

Description générale

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity. EGF binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue.

Immunogène

Paraformaldehyde-fixed carcinoma cell line A431

Application

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor specifically bind to a carbohydrate residue on the external region of the EGF receptor molecule with no interference to the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to the EGF Receptor. Reactivity has been observed with EGF receptors from mouse, human and bovine origin. The antibody is recommended to use in various immunological techniques, including Immunobloting (~170 kDa), Immunoprecipitation, flow cytometry (FACS), Immunohistochemistry and Immunofluorescence.

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Autres remarques

This product is for R&D use only, not for drug, household, or other uses.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable