SAB4200798

Anti-GSDMD antibody, Mouse monoclonal

clone GSD-14, purified from hybridoma cell culture

• Synonyme(s) : Anti-Gasdermin domain-containing protein 1, Anti-Gasdermin-D
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: GSD-14, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: ~53 kDa
• Espèces réactives: human
• Concentration: ~1 mg/mL
• Technique(s): immunoblotting: 2-4 μg/mL using human Jurkat cell extract; immunofluorescence: 5-10 μg/mL using human A431 cells
• Isotype: IgG1
• Numéro d'accès UniProt: P57764
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GSDMD(79792)

Description

Description générale

GSDMD (gasdermin D) also known as DFNA5L or Gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in the upper gastrointestinal epithelium differentiating cells and has been suggested to act as a tumor suppressor since it is suppressed in high percentage of esophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gramnegative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1β processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that Caspase-4/11 specifically cleaves GSDMD after Asp275, leading to gasdermin-N domain that bears intrinsic pyroptosisinducing activity. 4 Furthermore, GSDMD-mediated pyroptosis plays an important role in mature IL-1β release without affecting its maturation.

Monoclonal Anti-GSDMD recognizes human GSDMD.

Monoclonal Anti-GSDMD (mouse IgG1 isotype) is derived from the GSD-14hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with synthetic peptide corresponding to the internal region of human GSDMD, conjugated to keyhole limpet hemocyanine (KLH). Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1). It belongs to the gasdermin protein family of epithelial proliferation regulators and is expressed in the upper gastrointestinal epithelium differentiating cells.

Immunogène

Synthetic peptide of human GSDMD, conjugated to KLH

Application

The antibody is recommended to use in various immunological techniques, including Immunoblot (∼53 kDa) and Immunofluorescence.

Actions biochimiques/physiologiques

Gasdermin D (GSDMD) is known to act as a tumor suppressor since it is suppressed in a high percentage of oesophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gram-negative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1 processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). Furthermore, GSDMD-mediated pyroptosis plays an important role in mature interleukin-1 (IL-1) release without affecting its maturation.

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Autres remarques

This product is for R&D use only, not for drug, household, or other uses.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3