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SAB4200798

Sigma-Aldrich

Anti-GSDMD antibody, Mouse monoclonal

clone GSD-14, purified from hybridoma cell culture

Synonyme(s) :

Anti-Gasdermin domain-containing protein 1, Anti-Gasdermin-D

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

GSD-14, monoclonal

Forme

buffered aqueous solution

Poids mol.

~53 kDa

Espèces réactives

human

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 2-4 μg/mL using human Jurkat cell extract
immunofluorescence: 5-10 μg/mL using human A431 cells

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... GSDMD(79792)

Description générale

GSDMD (gasdermin D) also known as DFNA5L or Gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in the upper gastrointestinal epithelium differentiating cells and has been suggested to act as a tumor suppressor since it is suppressed in high percentage of esophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gramnegative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1β processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that Caspase-4/11 specifically cleaves GSDMD after Asp275, leading to gasdermin-N domain that bears intrinsic pyroptosisinducing activity. 4 Furthermore, GSDMD-mediated pyroptosis plays an important role in mature IL-1β release without affecting its maturation.

Monoclonal Anti-GSDMD recognizes human GSDMD.
Monoclonal Anti-GSDMD (mouse IgG1 isotype) is derived from the GSD-14hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with synthetic peptide corresponding to the internal region of human GSDMD, conjugated to keyhole limpet hemocyanine (KLH). Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1). It belongs to the gasdermin protein family of epithelial proliferation regulators and is expressed in the upper gastrointestinal epithelium differentiating cells.

Immunogène

Synthetic peptide of human GSDMD, conjugated to KLH

Application

The antibody is recommended to use in various immunological techniques, including Immunoblot (∼53 kDa) and Immunofluorescence.

Actions biochimiques/physiologiques

Gasdermin D (GSDMD) is known to act as a tumor suppressor since it is suppressed in a high percentage of oesophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gram-negative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1 processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). Furthermore, GSDMD-mediated pyroptosis plays an important role in mature interleukin-1 (IL-1) release without affecting its maturation.

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Autres remarques

This product is for R&D use only, not for drug, household, or other uses.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Inflammatory caspase-related pyroptosis: mechanism, regulation and therapeutic potential for inflammatory bowel disease
Kayagaki N, et al.
Gastroenterology, 526(7575), 666-666 (2015)
Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling
Kayagaki N, et al.
Nature, 526(7575), 666-666 (2015)
Wan-ting He et al.
Cell research, 25(12), 1285-1298 (2015-11-28)
Inflammasome is an intracellular signaling complex of the innate immune system. Activation of inflammasomes promotes the secretion of interleukin 1β (IL-1β) and IL-18 and triggers pyroptosis. Caspase-1 and -11 (or -4/5 in human) in the canonical and non-canonical inflammasome pathways
Members of a novel gene family, Gsdm, are expressed exclusively in the epithelium of the skin and gastrointestinal tract in a highly tissue-specific manner
Tamura M, et al.
Genomics, 89(5), 618-629 (2007)
Caina Ma et al.
Virologica Sinica, 37(3), 445-454 (2022-05-06)
African swine fever (ASF) is a highly pathogenic swine infectious disease that affects domestic pigs and wild boar, which is caused by the African swine fever virus (ASFV). ASF has caused huge economic losses to the pig industry and seriously

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