SAB4200545

Anti-Parvalbumin antibody, Mouse monoclonal

clone PARV-19, purified from hybridoma cell culture

• Synonyme(s) : Monoclonal Anti-ALPHA-PARVALBUMIN, Monoclonal Anti-PALB1, Monoclonal Anti-PV, Monoclonal Anti-PVALB (includes EG:19293), Monoclonal Anti-Parv, Monoclonal Anti-Pva
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: PARV-19, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 12 kDa
• Espèces réactives: rat, human
• Concentration: ~1.0 mg/mL
• Technique(s): immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat cerebellum.; indirect immunofluorescence: 10-20 μg/mL using BLO-11 cells.; western blot: 1-2 μg/mL using mouse muscle extracts.
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PVALB(5816) ; rat ... PVALB(25269)

Description

Description générale

Monoclonal Anti-Parvalbumin (mouse IgG1 isotype) is derived from the hybridoma PARV-19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified frog muscle parvalbumin. Parvalbumin (PV) is a member of the family of Ca²⁺-binding molecules. It carries three helix-loop-helix EF-hand Ca²⁺ binding motifs, with the two C-terminal motifs.

Spécificité

Monoclonal Anti-Parvalbumin recognizes parvalbumin in a Ca2+ -ion dependent manner

Immunogène

purified frog muscle parvalbumin. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Anti-Parvalbumin antibody, Mouse monoclonal has been used in:

• immunohistochemistry
• immunofluorescence staining
• immunolabeling
• to label brain slices for perineuronal net staining
• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

Ca²⁺ participates in cell motility, release of neurotransmitters, membrane permeability, excitation−contraction of muscles, cell secretory procedures, and transmission of nerve impulses. Intracellular Ca²⁺ exchangers are essential to modulate these processes efficiently. Parvalbumin (PV), a calcium-binding protein, can regulate the intracellular calcium dynamics in neurons. Hence it may have an impact on facilitation and depression at synapses. Interestingly, PV deficiency alters neuronal activity, a key mechanism leading to epileptic seizures or Parkinson.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable