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RPROB

Sigma-Aldrich

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)

Niveau de qualité

200

Technique(s)

protein staining: suitable

Température de stockage

2-8°C

Application

R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.

Autres remarques

Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.

Composants de kit seuls

Réf. du produit
Description
  • Solution A 500 mL
  • Solution B 500 mL

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290,H315,H319

Classification des risques

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)

Articles

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protocoles

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

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