RAB0178
Human Fas Ligand ELISA Kit
for serum, plasma, and cell culture supernatants
Synonyme(s) :
FasL
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About This Item
Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32
Produits recommandés
Espèces réactives
human
Conditionnement
kit of 96 wells (12 strips x 8 wells)
Technique(s)
ELISA: suitable
capture ELISA: suitable
Entrée
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.37-1000 pg/mL
Méthode de détection
colorimetric
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... FASLG(356)
Catégories apparentées
Description générale
The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine.
Immunogène
Recombinant Human Fas Ligand
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Actions biochimiques/physiologiques
Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS).
Autres remarques
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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Réf. du produit
Description
FDS
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Met. Corr. 1
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
W C Powell et al.
Current biology : CB, 9(24), 1441-1447 (1999-12-23)
The Fas ligand/Fas receptor (FasL/Fas) system is an important mediator of apoptosis in the immune system where the juxtaposition of cells expressing the cell-surface ligand induces the apoptotic pathway in Fas-expressing lymphocytes. The FasL/Fas system has also been shown to
M R Alderson et al.
The Journal of experimental medicine, 181(1), 71-77 (1995-01-01)
A significant proportion of previously activated human T cells undergo apoptosis when triggered through the CD3/T cell receptor complex, a process termed activation-induced cell death (AICD). Ligation of Fas on activated T cells by either Fas antibodies or recombinant human
O Micheau et al.
The Journal of biological chemistry, 274(12), 7987-7992 (1999-03-13)
Trimerization of the Fas receptor (CD95, APO-1), a membrane bound protein, triggers cell death by apoptosis. The main death pathway activated by Fas receptor involves the adaptor protein FADD (for Fas-associated death domain) that connects Fas receptor to the caspase
Yin Li et al.
International journal of clinical and experimental pathology, 8(9), 11915-11920 (2015-12-01)
To investigate the relation of Fas and Fas ligand (FasL) protein expression with carcinogenesis and metastasis of cardiac carcinoma. Immunohistochemistry was used to detect Fas and FasL protein expression in 64 cardiac carcinoma tissue samples and 20 normal gastric tissue
Çağman Tan et al.
The Turkish journal of pediatrics, 57(2), 141-145 (2015-12-23)
Defects in genes that have role in apoptotic pathways result in development of Autoimmune Lymphoproliferative Syndrome (ALPS) and ALPS related disorders. Germline and somatic FAS mutations, FASL and CASP10 mutations constitute other genetic defects in ALPS. Patients who fulfill ALPS
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