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Key Documents

R8903

Sigma-Aldrich

Monoclonal Anti-hnRNP-K/J antibody produced in mouse

clone 3C2, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Anti-Heterogeneous Nuclear Ribonucleoprotein K/J

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

3C2, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~60 kDa

Espèces réactives

hamster, Xenopus, bovine, human

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of MBDK cells

Isotype

IgG2b

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HNRNPK(3190)

Description générale

Monoclonal Anti-hnRNP-K/J (mouse IgG2b isotype) is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with hnRNP′s purified by oligo (dC) affinity chromatography.

Immunogène

hnRNPs purified by oligo (dC) affinity chromatography.

Application

The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).

Actions biochimiques/physiologiques

Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-K is found both in the nucleus, cytoplasm, and mitochondria, therefore, it was found to be involved in many biological events in the cell like: transcription, chromatin remodeling, splicing and translation. hnRNP-K is regulated by different signaling cascades and can bind kinases. As a consequence, it can mediate signals from the cytoplasm to the nucleus.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

hnRNP K: One protein multiple processes
Bomsztyk K, et al.
Bioessays, 26(6), 629-638 (2004)
Heterogeneous nuclear ribonucleoprotein K upregulates the kinetochore complex component NUF2 and promotes the tumorigenicity of colon cancer cells
Sugimasa H, et al.
Biochemical and Biophysical Research Communications, 459(1), 29-35 (2015)
A Proteomic Approach To Identify Candidate Substrates of Human Adenovirus E4orf6-E1B55K and Other Viral Cullin-Based E3 Ubiquitin Ligases
Dallaire F, et al.
Journal of Virology, 83(23), 12172-12184 (2009)
Cheng Zhang et al.
Arteriosclerosis, thrombosis, and vascular biology, 27(8), 1760-1767 (2007-05-05)
Inflammation is known to contribute to the pathogenesis of vascular diseases in which arterial wall extracellular matrix (ECM) homeostasis is disrupted. Tumor necrosis factor-alpha (TNF-alpha), a pivotal cytokine that regulates ECM metabolism by increasing degradation and decreasing production of arterial
SMN interacts with a novel family of hnRNP and spliceosomal proteins
Mourelatos Z, et al.
The Embo Journal, 20(19), 5443-5452 (2001)

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